Tuesday, October 4, 2022

Lactobacillus Rhamnosus Gr 1 Probiotic

How Chr Hansen Created Urex

fembio capsule in hindi – lactobacillus rhamnosus GR-1 and lactobacillus reuteri RC14 capsule

The Lactobacillus rhamnosus GR-1® strain was isolated in 1980 from distal urethra of a healthy woman while the Lactobacillus reuteri, RC-14® strain was isolated in 1985 from the vagina of a healthy woman. The probiotic strains have since been vigorously investigated in studies ranging from lab studies to randomized double-blinded, placebo-controlled trials. The first trial on the GR-1® strain was published in 1988, and the first trial on the RC-14® strain published in 2001. More than 30 trials have been published using the GR-1® and RC-14® strains in combination.

How Many Live Active Cultures And Strains Does It Contain

Culturelle® probiotics in Canada contain between 5 billion and 10 billion colony forming units of Lactobacillus rhamnosus GG. The amount of active cultures of the strain provided are clinically determined based on the benefits that are sought.

The Culturelle® Probiotic Advantage:

Culturelle® guarantees the stated number of live, naturally sourced Lactobacillus rhamnosus GG cultures through the expiration date on the package.** Culturelle® probiotic formulations are based on science, with just the right amount of live active cultures to support the unique benefits of each product.

Growth Media And Culture Conditions

Both probiotic lactobacilli strains were routinely cultured on de Man, Rogosa and Sharpe agar and incubated anaerobically for 48 h at 37°C. Subsequently, lactobacilli strains were inoculated into MRS broth and incubated anaerobically for 24 h at 37°C in an orbital shaker . All of the C. glabrata strains were cultured on Yeast Extract-Peptone-Dextrose agar and incubated aerobically for 24 h at 37°C. Candida glabrata colonies were transferred into YPD broth and incubated aerobically for 24 h at 37°C in an orbital shaker .

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Where To Find Lactobacillus Rhamnosus Gr

L. rhamnosus GR-1 is available, combined with Lactobacillus reuteri RC-14, in products such as Fem-Dophilus® and RepHresh Pro-B®. Always read ingredients prior to purchasing.

In Fem-Dophilus® , the recommended dosage is one capsule per day for maintenance with 2.5 billion cfu of each strain per capsule. Increase to two capsules a day as needed, or use as directed by your qualified health care consultant. Fem-Dophilus® is marketed by Jarrow and is recommended to be refrigerated.

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Has The Probiotic Strain Been Tested And Researched

Fem

Clinical studies and research are what distinguish promises from results. Be sure your probiotic is thoroughly studied, tested, and proven. To meet the gold standard, the research should be conducted in humans.

The Culturelle® Probiotic Advantage:

The probiotic strain in Culturelle® – Lactobacillus rhamnosus GG is the #1 most clinically studied strain of probiotic. Considered the premier probiotic in the world, over 1,000 scientific studies and over 30 years of research have demonstrated the safety and efficacy of Lactobacillus rhamnosus GG . It is able to survive harsh stomach acid and support a strong barrier against non-beneficial bacteria on the intestinal wall, providing a digestive health advantage.

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Probiotic Lactobacillus Rhamnosus Gr

  • Siwen YangAffiliationsDepartments of Physiology and Obstetrics and Gynecology, University of Toronto Faculty of Medicine, Toronto, ON, CanadaLunenfeldTanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
  • Wei LiAffiliationsDepartments of Physiology and Obstetrics and Gynecology, University of Toronto Faculty of Medicine, Toronto, ON, CanadaLunenfeldTanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
  • John R.G. ChallisAffiliationsDepartments of Physiology and Obstetrics and Gynecology, University of Toronto Faculty of Medicine, Toronto, ON, CanadaFaculty of Health Sciences, Simon Fraser University, Vancouver, BC, Canada
  • Department of Microbiology and Immunology, Western University Faculty of Medicine, London, ON, CanadaLawson Health Research Institute, London, ON, Canada
  • Alan D. BockingCorrespondenceReprints: Alan D. Bocking, MD, Department of Obstetrics and Gynecology, Mount Sinai Hospital Room 19-308, 600 University Avenue, Toronto, ON, Canada, M5T 1X5.Departments of Physiology and Obstetrics and Gynecology, University of Toronto Faculty of Medicine, Toronto, ON, CanadaLunenfeldTanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

Role In Maintaining Vaginal Health

Since a vaginal microbiota is dominated by Lactobacillus species in the majority of healthy women, the idea of administering Lactobacillus probiotics to restore and/or maintain health was perpetuated. The intent was not to colonize the niche but to use strains that would restore the health status of the area. Various studies have been performed to investigate the role of LGR-1 for the treatment and prevention of vaginal disorders (which has had almost no new therapeutic approaches in over 40

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The Worlds Most Documented Probiotics For Womens Urogenital Health

A combination of the Lactobacillus rhamnosus GR-1® and Lactobacillus reuteri RC-14® , the UREX strain combination has been associated with support of the female urogenital tracts of teens, premenopausal and post-menopausal women. The UREX combination is the worlds most documented probiotics for womens urogenital health.

Vaginal And Faecal Microbiota Sample Processing And Analysis

Probiotics for Women’s Health – Chapter 10

At enrolment and each follow-up time point, two vaginal samples were collected by specimen collection swabs Technology Co., Ltd., Guangdong, China): one for the screening of BV by Nugent scoring and to measure vaginal pH, and the other was stored in 3 ml of buffer Technology Co., Ltd, Guangdong, China) at -80°C and later used for vaginal microbiota DNA profiling. All the patients were asked to self-collect a sample of faeces at enrolment and each follow-up visit using a self-collection bag containing the transport medium from BGI. The faecal samples were also stored at -80°C immediately upon receipt at the laboratory.

Total DNA of vaginal and faecal samples was extracted using a QIAamp DNA Mini Kit and DNA concertation was determined by a Qubit Fluorometer, and the purity and integrity of the gDNA were measured by 0.6% agarose gel electrophoresis. The DNA samples were stored at -80°C for further usage. Qualified genomic DNA samples were configured with the specific primers 8F and 518R , and the variable regions V1 to V3 of the 16S rRNA gene were amplified by PCR. Subsequently, Agencourt AMPure XP magnetic beads were used to purify PCR amplicons, which were dissolved in TE buffer and labelled to complete library construction. An Agilent 2100 Bioanalyzer was used to detect the insert fragment size, and then the products were sequenced on the HiSeq2500 platform using the PE300 module.

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What Does Research On L Rhamnosus Gr

Some studies show that it is very helpful for a vaginal infection such as a vaginal yeast infection or bacterial vaginosis, and also for a urinary tract infection .

For more information on the specifics of the probiotic Lactobacillus rhamnosus GR-1, including details of what studies have shown for the strain and at which dosages and duration, look for a future publication, “The Lactobacillus rhamnosus GR-1 Guide.”

Can Lactobacillus Rhamnosus Gr

Lactobacillus rhamnosus GR-1 is a lactic acid bacteria from the genus Lactobacillus and the species rhamnosus. Like other Lactobacillus rhamnosus, it is a Gram-positive, rod-shaped bacterium which does not produce spores and is not capable of moving itself with flagella.

L. rhamnosus GR-1 was isolated from the urethra of a healthy woman. The urethra is a tube that connects the urinary bladder to the urinary meatus for the removal of fluids from a woman’s body. Dr. Gregor Reid and Dr. Andrew Bruce at Urex Biotech discovered and developed Lactobacillus rhamnosus, GR-1® and then sold the rights to Chr. Hansen of Denmark.

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Probiotic Lacticaseibacillus Rhamnosus Gr

  • 1Department of Obstetrics and Gynaecology, Peking University Shenzhen Hospital, Shenzhen, China
  • 2Institute of Obstetrics and Gynecology, Shenzhen PKU-HKUST Medical Center, Shenzhen, China
  • 3Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynaecological Disease, Shenzhen, China
  • 4College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
  • 5BGI-Shenzhen, Shenzhen, China

What Are The Benefits Of Lactobacillus Rhamnosus

Lactobacillus Rhamnosus pack of 60 capsules *  Epigenetics

Lactobacillus rhamnosus has been the focus of many research projects with some strains showing to be particularly beneficial for supporting female intimate health conditions. These certain strains of L. rhamnosus support vaginal health in the following ways:

  • Colonising in the vaginal tract Colonising primarily in the vaginal tract rather than the intestines, therefore supporting vaginal health .
  • Attachment to the vaginal mucosa By adhering to the urethra, certain strains of L. rhamnosus will displace and prevent the adhesion of pathogens to the vaginal lining which protects against infections such as bacterial vaginosis .

You can read more about this in our article on our sister site, Probiotics Learning Lab: Probiotics and bacterial vaginosis

Lactobacillus rhamnosus also supports digestive and immune health in the following ways:

  • Production of antimicrobial substances This helps to alleviate diarrhoea that can be associated with travel or antibiotic use .
  • Reduction of inflammatory signalling in the intestines – Reducing the frequency of IBS and IBD related abdominal pain. .
  • Modulation of the immune system By increasing the action of T-lymphocytes and the action of anti-inflammatory cytokines. .

You can find more information on how probiotics can aid immune function in this article on the Probiotics Learning Lab: Could live cultures help allergies?

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Is L Rhamnosus Gr

The Lactobacillus rhamnosus species as a whole are listed on the European QPS list. Lactobacillus bacteria as a whole are considered to be routinely encountered in the environment, in fermented foods and drinks, and are frequently isolated from feces. Unless a person is immune-compromised, Lactobacillus are generally considered safe. If you ever have any questions about your immune status, please talk with your doctor.

Medical Research And Use

While L. rhamnosus GG is able to survive the acid and bile of the stomach and intestine, is claimed to colonize the digestive tract, and to balance intestinal microbiota, evidence suggests that L. rhamnosus, comparable to virtually all probiotic lactobacilli, is only a transient inhabitant and not autochthonous. Regardless, it is considered a probiotic useful for the treatment of various maladies, as it works on many levels. Lactobacillus rhamnosus GG binds to the gut mucosa.

Diarrhea

Lacticaseibacillus rhamnosus GG is beneficial in the prevention of rotavirusdiarrhea in children. Prevention and treatment of various types of diarrhea have been shown in children and in adults.L. rhamnosus GG can be beneficial in the prevention of antibiotic-associated diarrhea and nosocomial diarrhea and this has been recently supported by European guidelines.Lactobacillus rhamnosus GG may reduce the risk of traveler’s diarrhea.

Acute gastroenteritis

A position paper published by ESPGHAN Working Group for Probiotics and Prebiotics based on a systematic review and randomized controlled trials suggested that L. rhamnosus GG may be considered in the management of children with acute gastroenteritis in addition to rehydration therapy.

Atopic dermatitis, eczema

Risks

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Evaluation Of The Key Species

Figure 5 The abundance of the top abundant bacterial species and key Lactobacillus species in vaginal microbiota. The relative abundances of bacterial species with abundance above 0.01 in the vaginal environment of BV patients have been evaluated . The relative abundance of vaginal dominant Lactobacillus species , including L. crispatus, L. gasseri and L. iners, as well as the intervention probiotic species in the vaginal microbiota have also been explained. Wilcoxon test was used to do the statistical analysis of each two independent groups, and p-value was noted by stars above the bars. *stands for p-value < 0.05 **stands for p-value < 0.01 ***stands for p-value < 0.001.

Protein Extraction And Cytokine/chemokine Multiplex Assay

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Vaginal swab samples were equilibrated in Tris-HCl buffer with 150 mmol/L NaCl, 1 mmol/L phenylmethylsulfonyl fluoride , 0.05% Tween-20 and a protease inhibitor cocktail tablet for 30 min at 4 °C and vortexed every 10 min. The swab was removed, and buffer was centrifuged at 16,000Ãg for 15 min at 4 °C. Supernatant was stored at â80 °C in aliquots until further analysis. IL-1 receptor antagonist , IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, basic Fibroblast Growth Factor , Colony Stimulating Factor 2, CSF3, Interferon -γ, CXCL10, CCL2, CCL3, CCL4, CCL5, CCL11, Platelet-Derived Growth Factor -bb, Tumor Necrosis Factor -α and Vascular Endothelial Growth Factor were measured with a 27 human multiplex cytokine/chemokine kit .

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Growth Inhibitory Activity Of Probiotic Lactobacilli Strains Against Candida Glabrata

The growth inhibitory activity of probiotic lactobacilli strains was demonstrated as both Lact. rhamnosus GR-1 and Lact. reuteri RC-14 strains produced visible inhibition zones against all of the tested C. glabrata strains. In addition, C. glabrata ATCC 2001 was the most sensitive strain to the growth inhibitory effects of Lact. rhamnosus GR-1 and Lact. reuteri RC-14, with the largest inhibition zones of 1·63 ± 0·04 and 1·54 ± 0·04, respectively .1). To investigate whether pH contributes to the growth inhibitory effects of probiotic lactobacilli strains, the pH of MRS agar was buffered and neutralized by the addition of MOPS to pH 7·0. The probiotic Lact. rhamnosus GR-1 and Lact. reuteri RC-14 strains still managed to produce visible inhibition zones against all of the tested C. glabrata strains despite the buffering of the growth media with MOPS to a pH of 7·0 before inoculation. However, the visible inhibition zones appeared to be smaller than those of the previous experiments that utilized unbuffered MRS agar .1). The growth inhibitory activity of Lact. rhamnosus GR-1 and Lact. reuteri RC-14 against the tested C. glabrata strains was reduced by 2356% and 2062%, respectively, when the MRS media were neutralized to pH 7·0.

Healthy Vaginal Bacterial Balance

Some studies have associated UREXTM better outcomes for subjects treated for bacterial infections with antibiotics combined with UREXTM compared to subjects treated with an antibiotic combined with placebo2, 6, 10

Also, some studies have associated a greater reduction in a clinical marker of bacterial infection in subjects supplemented with UREXTM alone compared with those treated with a placebo3, 4, 7

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The Urex Strain Combinationhelps Provide Relief From Bacterial Imbalances In The Urogenital Tracts

The UREX strain combination has been associated with beneficial effects across the female urogenital tracts in both teens, premenopausal and post-menopausal women. The UREX combination has been shown that it:

  • may provide relief from vaginal bacterial imbalance alone and in combination with standard health care intervention1-6
  • may help establish and maintain a healthy vaginal pH3
  • may help support a healthy vaginal microbiota following vaginal bacterial imbalance4
  • support balancing of the vaginal microbiota during challenges with yeast overgrowth8
  • may reduce vaginal discharge8
  • may increase the abundance of healthy lactobacilli in the vaginal tract9, 2
  • may reduce the number of times with challenges due to bacterial imbalance in the urinary tract.7

Bacterial Strains And Growth Conditions

Probiotique Lactobacillus Rhamnosus

L. rhamnosus GR-1 and GG were cultured on de Man Rogosa Sharp agar anaerobically using anaerobic packs at 37°C for 24 h under static condition. For cell culture challenge, lactobacilli were grown from a 1% inoculum in MRS broth for 24 h followed by washing and resuspending in the original volume with phosphate buffered saline . Uropathogenic E. coli GR12 was grown in Luria-Bertani medium at 37°C and constant shaking. Heat-killed bacteria were prepared by washing cultures in PBS and heating at 70°C for 1 h followed by plating 100 l on the respective growth medium to confirm loss of viability. Heat-killed L. rhamnosus GR-1 and E. coli were stored at -20°C until used for cell challenge.

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Study Groups And Randomization

A total of 328 women between 12- and 16 weeks of gestation consented and were screened between May and October for the presence of an intermediate or high Nugent score . Eighty-six women whose vaginal samples had a Nugent score ⥠4 were randomized.

Consort flow chart of pregnant women enrolled in the study.

A Z test was used to determine the sample size with alpha = 0.05 and power = 0.8, giving a per-group sample size of 40 women to detect a difference between an asymptomatic Intermediate/BV prevalence of 30% in the probiotic group and 60% in the placebo group at the end of treatment protocol. This was increased to 43 in each group to compensate for 5% lost to follow-up. Subjects were randomized using a web-based service to receive two identical looking capsules per day containing either GR-1 and RC-14 or placebo given orally for 12 weeks. Vaginal swabs were collected at 13-, 28- and 35 weeks of gestation and analyzed for Nugent score, cytokines, chemokines and microbiota. Fourteen subjects were lost to follow-up or withdrew, 3 had taken less than 25% of the capsules, and there was insufficient sample for analysis in 3 others. This left 32 subjects in the probiotic and 34 in the placebo group with samples available for sequencing analysis and samples from 31 probiotic and 33 placebo subjects for cytokines and chemokine analysis.

Dna Isolation And Polymerase Chain Reaction Amplification Of V6 Region Of 16s Rdna

Vaginal swabs were equilibrated in 800μL phosphate buffer saline on ice and vortexed for 1 min. DNA was extracted with a Qiagen Stool Extraction Kit , bacterial DNA was amplified with barcoded primers targeting the V6 region of the 16S rDNA and PCR amplification was performed with colorless GO-Taq hot start master mix for 25 repeating cycles of 95 °C, 55 °C and 72 °C for 1 minute each step. The amplified products were quantified using a QuBit broad-range double-stranded DNA fluorometric quantitation reagent kit . Samples were pooled at equal molar concentrations and purified using Wizard PCR Clean-Up Kits .

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The Association Of Intervention Outcome With The Microbiota Present Before Treatment

Figure 6 The hierarchical cluster of the vaginal and fecal microbiota of the participates at 0-day. The heatmap of the hierarchical cluster analysis of the bacterial species with relative abundance above 0.001 in vaginal and fecal microbiota of each participants. The colour was corresponded with the colour bar at right side defined by log of the relative abundance. The left bar was represented the cure and non-cure outcome of the participants. The relative abundance of the bacterial species above 0.01 in vaginal microbiota in cure group and non-cure group, and their multiple relationship were also calculated . The number marked at the end of the bar in the upper plot was the mean relative abundance, and the number in the lower plot was the fold change. Wilcoxon test was used to do the statistical analysis of each two independent groups, and p-value was noted by stars above the bars. *stands for p-value < 0.05 **stands for p-value < 0.01.

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